stem-cells-training-researchers-human-iPSCs

The Center provides users with a clean, dedicated space specifically for culturing human induced pluripotent stem cells (iPSCs), occupying approximately 1,250 square feet of space on the second floor of Porter Biosciences, on the CU Boulder main campus.听

We provide:

  • 5听Biosafety Cabinets
  • 7 CO2听and tri-gas听incubators
  • Automated Fluorescence Cell Counter
  • Inverted Fluorescence Scanner Microscope (Olympus IX83)听with live-cell time-lapse capabilities听(Live and fixed cell imaging in monolayer or suspensions, organoids, embryos, organotypic slides, and more!)
  • Sterile Dissection Hood equipped with a dissecting Olympus CKX53 microscope and heated/CO2听stage for work with live cultures and live/fix imagine
  • Olympus听Provi听CM20听(imaging and monitoring cell confluence, cell count, and cell culture data records)
  • EVOS7000 Microscope
  • CO2 shaker + Remote Controller for Organoids Derivation
  • Transfection Systems (Neon NxT Electroporation &听Amaxa听Nucleofector)
  • Phase Contrast Microscopes听
  • Liquid Nitrogen Cryostorage Space
  • 6 Water/Beads Bath and听ThawSTAR听Automated Cell Thawing System
  • Centrifuges and Microcentrifuges
  • Plate shakers
  • Block heathers (microcentrifuge tubes)
  • Mr. Frosty听&听CoolCell听听LX Cell Freezing Containers
  • Bioengineering Space听
  • Standard iPSC consumables (i.e., pipettes, plasticware, media, substrates, etc.) are readily available to users.听
  • Reagents for sale (even 30% less)听
  • Training of new users
  • hiPSC training. 听 ***Coming soon: Neural Culture workshop!
  • Microscope training
  • Refrigerators &听freezers (4OC, -20oC, -80oC)

Stem cell center user using a microscope to inspect cells.

听