A new University of Colorado Boulder study has shown that some dividing human cells are 鈥渒icking the can down the road,鈥� passing on low-level DNA damage to offspring, causing daughter cells to pause in a quiescent, or dormant, state previously thought to be random in origin.
The process occurs even among genetically identical cells grown under identical, optimal conditions, said CU Boulder Assistant Professor Sabrina Spencer, principal investigator on the study. The study indicates some offspring inherit not only DNA damage, but also听a lengthened cell-quiescent period the more damage there is.
鈥淲e have linked DNA damage in mother cells to the fate of daughter cells in the subsequent cell cycle,鈥� said Spencer of the . 鈥淭he study shows that entry into this quiescent state is not random, but rather is influenced by a 鈥榤emory鈥� of events that occurred in the previous generation or generations.鈥�
The study appears in the May 16 issue of Cell Reports.
Several years ago, Spencer developed a new technology using fluorescent biosensors that allow her team to identify, isolate and characterize quiescent cells in real time. With the ability to dynamically track changes in such cells, it may now be possible to determine to what extent a 鈥渂ypass鈥� of the quiescent state by cells in the presence of stress could be a precursor or even a hallmark of cancer, she said.
鈥淪uch findings could contribute to the development of biomarkers to identify cancer cells that may be particularly sensitive to chemotherapeutic agents due to their inability to enter quiescence in response to stress,鈥� the team wrote in Cell Press.
The fact that mother cells are passing on DNA damage to their offspring was a surprising finding because the researchers expected mother cells would repair their own damage before dividing into daughter cells, said Spencer.
鈥淚n our world and the cellular world, we would think mothers would deal with their problems and not pass them on to their offspring,鈥� Spencer said. 鈥淐learly this is not happening in many of the cells we have studied.鈥�
The new study also may have implications for aging since some quiescent daughter cells with unrepaired DNA damage may move into a senescent state鈥攅ssentially the loss of a cell鈥檚 power to undergo division and growth, said Spencer.
Study co-authors include postdoctoral researcher Mansi Arora and doctoral student Justin Moser of chemistry and biochemistry and former master鈥檚 students Harsha Phadke and Ashik Akbar Basha of the
The study was funded primarily by awards to Spencer, including a National Institutes of Health K22 Early Career Investigator Award, a Boettcher Webb-Waring Early Career Investigator Award, A Kimmel Scholar Award, a Searle Scholar award and a Beckman Young Investigator Award.
